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Does the PCIe x16 small slot need to be occupied?

This may be a silly question but I’m asking in desperation as I don’t want to return my RAID controller card (I’m finding them difficult to source as i need PCIe 3.0 and hardware RAID).

I stupidly asked for a PC to be built by gamers and I received a small motherboard with very little room to manoeuvre.

I have an ASUS TUF Gaming B550M-PLUS motherboard and I bought a HPE Smart Array E208i-p SR Gen10 Storage Controller RAID. The size of the controller means that the small connector will not fit into the small slot on the PCIe 16 port. Will the card work if I plug the card into the longer lane and I leave the small lane unoccupied?

If I try plugging it in the suppliers said I can’t return it.