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Install Win10 on new drive, avoid conflict with existing C partition

I have a Dell XPS 8930 with two SSDs, 1TB and 500GB. The current Win10 install is on the 1TB in a “C” partition. I also have most of my user data on the 1TB on different partitions (all safely backed up). The 500GB contains only disposable junk data.

As part of a complex diagnostic procedure, I’m going to unplug the 1TB and install a new Win10 on the 500GB. I’m assuming that will clear the 500GB drive and install the OS on a new “C” partition on the 500GB.

When I re-plug-in the 1TB, the system will “see” the original C. Will there a conflict with the new C partition on the 500GB? Or will the system just assign the “old” C to an available drive letter? If there is a conflict, how can I resolve that?

I do not want to change the new OS partition to another drive letter, to ensure that all drivers work, and to avoid any other such problems.

I can move the 1TB to an external USB enclosure and look at it on another computer, possibly re-letter the C drive on the 1TB from that other computer, there, however, it seems like that’s going to run into the same issue.

Again, will there be a conflict, and if so, what can I do to resolve it?