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How to defragment Virtual Machine in order to optimise virtual HD backups?

I’ve got the following setup:

  • a virtual machine with a virtual HDD at 160GB split over around 50 files (it’s a VMWare VM, so I’m talking about VMDK files)
  • after working in the VM for some hours, my daily backup will copy all 50 file, because they all have modifications

My question is this:

  • Is there a tool to “defragment” the windows file system in a way to keep the busy files roughly together?
  • (The idea is that fewer HDD files are modified in order to improve backup performance)

My questions are not:

  • How to defragment a HDD in general…
  • How to improve backup performance in general…
  • I’m not looking for suggestions on backup software…